Physicochemical and immunological assessment of engineered pure protein particles with different redox states

KT Gause; Y Yan; J Cui; NM Obrien-Simpson; JC Lenzo; EC Reynolds; F Caruso

Journal article

Physicochemical and immunological assessment of engineered pure protein particles with different redox states

KT Gause, Y Yan, J Cui, NM Obrien-Simpson, JC Lenzo, EC Reynolds, F Caruso

ACS Nano | AMER CHEMICAL SOC | Published : 2015

DOI: 10.1021/acsnano.5b00393

Download PDF

Abstract

The development of subunit antigen delivery formulations has become an important research endeavor, especially in cases where a whole cell vaccine approach has significant biosafety issues. Particle-based systems have shown particular efficacy due to their inherent immunogenicity. In some cases, fabrication techniques can lead to changes in the redox states of encapsulated protein antigens. By employing a uniform, well-characterized, single-protein system, it is possible to elucidate how the molecular details of particle-based protein antigens affect their induced immune responses. Using mesoporous silica-templated, amide bond-stabilized ovalbumin particles, three types of particles were fab..

View full abstract

University of Melbourne Researchers

Neil O'Brien-Simpson Author

Eric Reynolds Author

Frank Caruso Author

Related Projects (3)

ARC CENTRE OF EXCELLENCE IN CONVERGENT BIO–NANO SCIENCE AND TECHNOLOGY

Role of T cell in Chronic Periodontitis

Particular immune cell populations (innate T cells), have a critical role in immunity. These cells are stimulated by bacterial cell wall com..

ENGINEERING MATERIALS FOR ADVANCES IN NANOMEDICINE

Nanomedicine is one of the fastest growing areas in nanotechnology. This project will develop next-generation particle systems with engineer..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

This work was supported by the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030), Discovery Early Career Researcher Award (Y.Y., DE130100488), and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036) schemes, as well as the National Health and Medical Research Council Project Grant (APP1029878). K.T.G. acknowledges funding from the Australian Government through an International Postgraduate Research Scholarship and an Australian Postgraduate Award.